Dynamics of Protein Folding and Subunit Interactions in Assembly of the Yeast Mediator Complex
نویسنده
چکیده
..................................................................................7 LIST OF ABBREVIATIONS.......................................................8 LIST OF PAPERS.........................................................................9 PART 1: INTRODUCTION.......................................................10 I. EUKARYOTIC TRANSCRIPTION .............................................................. 10 II. TRANSCRIPTIONAL COFACTORS .......................................................... 14 III. YEAST AS A MODEL SYSTEM............................................................... 15 IV. YEAST MEDIATOR ............................................................................. 16 A. Discovery ......................................................................................... 16 B. Composition of Mediator ................................................................. 17 C. The New Nomenclature for Mediator .............................................. 18 D. Domains and Subcomplexes ............................................................ 20 E. Mediator Conservation during Evolution........................................ 23 V. OVERVIEW: PROTEIN COMPLEX ASSEMBLY ........................................ 24 A. Ribosome and Spliceosome Assemblies ........................................... 24 B. Nucleosome Assembly...................................................................... 25 VI. AIMS.................................................................................................. 26 PART 2: SUMMARY OF PAPERS ..........................................27 I. FUNCTIONAL AND PHYSICAL INTERACTIONS WITHIN THE MIDDLE DOMAIN OF THE YEAST MEDIATOR (PAPER I) ........................................................... 27 II. MED8, MED18, AND MED20 SUBUNITS OF THE MEDIATOR HEAD DOMAIN ARE INTERDEPENDENT UPON EACH OTHER FOR FOLDING AND COMPLEX FORMATION (PAPER II) ............................................................... 28 PART 3: CONCLUSION............................................................30 ACKNOWLEDGEMENTS........................................................32 REFERENCE LIST ....................................................................34
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